Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis

Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes bimodal consisting few pairs macrochromosomes and many microchromosomes. Although the is typically 2n = 80, diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed understand extent conservation karyotype’s organizational structure within four group using Bacterial Artificial Chromosomes via Fluorescence Situ Hybridization (BAC-FISH) microchromosome probes Chicken (Gallus gallus) or Zebra Finch (Taeniopygia guttata) per microchromosomes (GGA10-28, except GGA16). By examining chromosome complement passerine species—the Streaked Flycatcher (Myiodynastes maculatus), Shiny Cowbird (Molothrus bonariensis), Southern House Wren (Troglodytes aedon), Double-collared Seedeater (Sporophila caerulescens)—we discovered new Wren. Through FISH experiments, were able observe same pattern organization as in common ancestor birds. As result, propose confirm status organization, may confer evolutionary advantages group.